Science, Tech, Math › Science 10X TAE Electrophoresis Buffer Share Flipboard Email Print Andrew Brookes / Getty Images Science Chemistry Basics Chemical Laws Molecules Periodic Table Projects & Experiments Scientific Method Biochemistry Physical Chemistry Medical Chemistry Chemistry In Everyday Life Famous Chemists Activities for Kids Abbreviations & Acronyms Biology Physics Geology Astronomy Weather & Climate By Anne Marie Helmenstine, Ph.D. Chemistry Expert Ph.D., Biomedical Sciences, University of Tennessee at Knoxville B.A., Physics and Mathematics, Hastings College Dr. Helmenstine holds a Ph.D. in biomedical sciences and is a science writer, educator, and consultant. She has taught science courses at the high school, college, and graduate levels. our editorial process Facebook Facebook Twitter Twitter Anne Marie Helmenstine, Ph.D. Updated September 10, 2018 This is the protocol or recipe for preparing 10X TAE electrophoresis buffer: 10X TAE Electrophoresis Buffer Materials 48.4 g of Tris base [tris(hydroxymethyl)aminomethane]11.4 mL of glacial acetic acid (17.4 M)3.7 g of EDTA, disodium saltdeionized water Prepare the 10X TAE Electrophoresis Buffer Dissolve the Tris, glacial acetic acid and EDTA in 800 ml of deionized water.Dilute the buffer to 1 L. You do not need to sterilize the solution. 10X TAE Electrophoresis Buffer Storage Store the bottle of 10X buffer solution at room temperature. Using 10X TAE Electrophoresis Buffer The solution is diluted before use. Dilute 100 mL of 10X stock to 1 L with deionized water.