10X TAE Electrophoresis Buffer

Recipe for TAE Buffer

TRIS or THAM is the abbreviation for the organic compound tris(hydroxymethyl)aminomethane.
TRIS or THAM is the abbreviation for the organic compound tris(hydroxymethyl)aminomethane, a compound widely used in biochemistry to prepare buffer solutions. Klaus Hoffmeier

This is the protocol or recipe for preparing 10X TAE electrophoresis buffer:

10X TAE Electrophoresis Buffer Materials

  • 48.4 g of Tris base [tris(hydroxymethyl)aminomethane]
  • 11.4 mL of glacial acetic acid (17.4 M)
  • 3.7 g of EDTA, disodium salt
  • deionized water

Prepare the 10X TAE Electrophoresis Buffer

  1. Dissolve the Tris, glacial acetic acid and EDTA in 800 ml of deionized water.
  2. Dilute the buffer to 1 L. You do not need to sterilize the solution.

    10X TAE Electrophoresis Buffer Storage

    Store the bottle of 10X buffer solution at room temperature.

    Using 10X TAE Electrophoresis Buffer

    The solution is diluted before use. Dilute 100 mL of 10X stock to 1 L with deionized water.

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    Your Citation
    Helmenstine, Anne Marie, Ph.D. "10X TAE Electrophoresis Buffer." ThoughtCo, Mar. 24, 2017, thoughtco.com/10x-tae-electrophoresis-buffer-608131. Helmenstine, Anne Marie, Ph.D. (2017, March 24). 10X TAE Electrophoresis Buffer. Retrieved from https://www.thoughtco.com/10x-tae-electrophoresis-buffer-608131 Helmenstine, Anne Marie, Ph.D. "10X TAE Electrophoresis Buffer." ThoughtCo. https://www.thoughtco.com/10x-tae-electrophoresis-buffer-608131 (accessed May 25, 2018).