Recipe for Crystal Violet for the Gram Stain

Recipe for Crystal Violet for the Gram Stain

Crystal violet stain
weegeebored/Flickr/CC BY-ND 2.0

This is the recipe for the crystal violet stain used in the Gram stain and other staining procedures.

Crystal Violet Ingredients

  • 2 g crystal violet
  • 20 ml 95% ethyl alcohol
  • 0.8 g ammonium citrate monohydrate
  • 80 ml deionized water

Prepare Crystal Violet Stain

  1. Dissolve 2 g crystal violet in 20 ml of 95% ethyl alcohol.
  2. Dissolve 0.8 g ammonium oxalate monohydrate in 80 ml deionized water.
  3. Mix the crystal violet and ammonium oxalate monohydrate solutions to make the crystal violet stain. Filter the stain if necessary.