Science, Tech, Math › Science How to Make Kastle-Meyer Solution Presumptive Test to Detect Blood Share Flipboard Email Print Rafe Swan / Getty Images Science Chemistry Basics Chemical Laws Molecules Periodic Table Projects & Experiments Scientific Method Biochemistry Physical Chemistry Medical Chemistry Chemistry In Everyday Life Famous Chemists Activities for Kids Abbreviations & Acronyms Biology Physics Geology Astronomy Weather & Climate By Anne Marie Helmenstine, Ph.D. Chemistry Expert Ph.D., Biomedical Sciences, University of Tennessee at Knoxville B.A., Physics and Mathematics, Hastings College Dr. Helmenstine holds a Ph.D. in biomedical sciences and is a science writer, educator, and consultant. She has taught science courses at the high school, college, and graduate levels. our editorial process Facebook Facebook Twitter Twitter Anne Marie Helmenstine, Ph.D. Updated June 04, 2020 The Kastle-Meyer test is a simple, reliable and inexpensive test to detect blood. Here is how to prepare the Kastle-Meyer solution used for the forensic test. Kastle-Meyer Solution Materials 0.1 g phenolphthalein powder25% w/v sodium hydroxide solution (aqueous)0.1 g mossy zincdistilled water70% ethanol Procedure In a test tube, dissolve 0.1 g phenolphthalein in 10.0 ml of 25% sodium hydroxide solution.Add 0.1 g mossy zinc to the tube. The solution should be bright pink.Add a boiling chip and gently boil the solution until it changes color to become colorless or pale yellow. Add water, as necessary, to maintain the volume during boiling.Allow the solution to cool. Decant the liquid and dilute it to 100 ml with 70 ethanol. This is the Kastle-Meyer solution.Store the solution in a tightly-capped blue or brown bottle. Sources Meyers, Thomas C. (2006). "Chapter 21: Serology". In Wecht, Cyril H.; Rago, John T. (eds.). Forensic Science and Law: Investigative Applications in Criminal, Civil, and Family Justice. Boca Raton, FL: CRC Press. pp. 410–412. ISBN 0-8493-1970-6.Remsen, Ira; Rouiller, Charles August (eds.) "Phenolphthalin as a reagent for the oxidizing ferments". American Chemical Journal. 26 (6) : 526–539.