Science, Tech, Math › Science Nitrogenous Bases - Definition and Structures Share Flipboard Email Print Nitrogenous bases are found in DNA and RNA. Shunyu Fan / Getty Images Science Chemistry Biochemistry Basics Chemical Laws Molecules Periodic Table Projects & Experiments Scientific Method Physical Chemistry Medical Chemistry Chemistry In Everyday Life Famous Chemists Activities for Kids Abbreviations & Acronyms Biology Physics Geology Astronomy Weather & Climate By Anne Marie Helmenstine, Ph.D. Chemistry Expert Ph.D., Biomedical Sciences, University of Tennessee at Knoxville B.A., Physics and Mathematics, Hastings College Dr. Helmenstine holds a Ph.D. in biomedical sciences and is a science writer, educator, and consultant. She has taught science courses at the high school, college, and graduate levels. our editorial process Facebook Facebook Twitter Twitter Anne Marie Helmenstine, Ph.D. Updated May 06, 2019 A nitrogenous base is an organic molecule that contains the element nitrogen and acts as a base in chemical reactions. The basic property derives from the lone electron pair on the nitrogen atom. The nitrogen bases are also called nucleobases because they play a major role as building blocks of the nucleic acids deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). There are two major classes of nitrogenous bases: purines and pyrimidines. Both classes resemble the molecule pyridine and are nonpolar, planar molecules. Like pyridine, each pyrimidine is a single heterocyclic organic ring. The purines consist of a pyrimidine ring fused with an imidazole ring, forming a double ring structure. 01 of 07 The 5 Main Nitrogen Bases Nitrogen bases bind to complementary bases in DNA and RNA. Shunyu Fan / Getty Images Although there are many nitrogenous bases, the five most important to know are the bases found in DNA and RNA, which are also used as energy carriers in biochemical reactions. These are adenine, guanine, cytosine, thymine, and uracil. Each base has what is known as a complementary base that it binds to exclusively to form DNA and RNA. The complementary bases form the basis for the genetic code. Let's take a closer look at the individual bases... 02 of 07 Adenine Adenine purine nitrogen base molecule. MOLEKUUL/SCIENCE PHOTO LIBRARY / Getty Images Adenine and guanine are purines. Adenine is often represented by the capital letter A. In DNA, its complementary base is thymine. The chemical formula of adenine is C5H5N5. In RNA, adenine forms bonds with uracil. Adenine and the other bases bond with phosphate groups and either the sugar ribose or 2'-deoxyribose to form nucleotides. The nucleotide names are similar to the base names but have the "-osine" ending for purines (e.g., adenine forms adenosine triphosphate) and "-idine" ending for pyrimidines (e.g., cytosine forms cytidine triphosphate). Nucleotide names specify the number of phosphate groups bound to the molecule: monophosphate, diphosphate, and triphosphate. It is the nucleotides that act as building blocks of DNA and RNA. Hydrogen bonds form between the purine and complementary pyrimidine to form the double helix shape of DNA or act as catalysts in reactions. 03 of 07 Guanine Guanine purine nitrogen base molecule. MOLEKUUL/SCIENCE PHOTO LIBRARY / Getty Images Guanine is a purine represented by the capital letter G. Its chemical formula is C5H5N5O. In both DNA and RNA, guanine bonds with cytosine. The nucleotide formed by guanine is guanosine. In the diet, purines are abundant in meat products, particularly from internal organs, such as liver, brains, and kidneys. A smaller amount of purines are found in plants, such as peas, beans, and lentils. 04 of 07 Thymine Thymine pyrimidine nitrogen base molecule. MOLEKUUL/SCIENCE PHOTO LIBRARY / Getty Images Thymine is also known as 5-methyluracil. Thymine is a pyrimidine found in DNA, where it binds to adenine. The symbol for thymine is a capital letter T. Its chemical formula is C5H6N2O2. Its corresponding nucleotide is thymidine. 05 of 07 Cytosine Cytosine pyrimidine nitrogen base molecule. LAGUNA DESIGN / Getty Images Cytosine is represented by the capital letter C. In DNA and RNA, it binds with guanine. Three hydrogen bonds form between cytosine and guanine in the Watson-Crick base pairing to form DNA. The chemical formula of cytosine is C4H4N2O2. The nucleotide formed by cytosine is cytidine. 06 of 07 Uracil Uracil pyrimidine nitrogen base molecule. MOLEKUUL/SCIENCE PHOTO LIBRARY / Getty Images Uracil may be considered to be demethylated thymine. Uracil is represented by the capital letter U. Its chemical formula is C4H4N2O2. In nucleic acids, it is found in RNA bound to adenine. Uracil forms the nucleotide uridine. There are many other nitrogenous bases found in nature, plus the molecules may be found incorporated into other compounds. For example, pyrimidine rings are found in thiamine (vitamin B1) and barbituates as well as in nucleotides. Pyrimidines are also found in some meteorites, although their origin is still unknown. Other purines found in nature include xanthine, theobromine, and caffeine. 07 of 07 Review Base Pairing PASIEKA / Getty Images In DNA the base pairing is: A - TG - C In RNA, uracil takes the place of thymine, so the base pairing is: A - UG - C The nitrogenous bases are in the interior of the DNA double helix, with the sugars and phosphate portions of each nucleotide forming the backbone of the molecule. When a DNA helix splits, like to transcribe DNA, complementary bases attach to each exposed half so identical copies can be formed. When RNA acts as a template to make DNA, for translation, complementary bases are used to make the DNA molecule using the base sequence. Because they are complementary to each other, cells require approximately equal amounts of purine and pyrimidines. In order to maintain a balance in a cell, production of both purines and pyrimidines is self-inhibiting. When one is formed, it inhibits the production of more of the same and activates production of its counterpart.