Science, Tech, Math › Science What Are the 3 Parts of a Nucleotide? How Are They Connected? How Nucleotides Are Constructed Share Flipboard Email Print A nucleotide consists of base, sugar, and phosphate group. ThoughtCo. Science Chemistry Biochemistry Basics Chemical Laws Molecules Periodic Table Projects & Experiments Scientific Method Physical Chemistry Medical Chemistry Chemistry In Everyday Life Famous Chemists Activities for Kids Abbreviations & Acronyms Biology Physics Geology Astronomy Weather & Climate By Anne Marie Helmenstine, Ph.D. Anne Marie Helmenstine, Ph.D. Facebook Twitter Chemistry Expert Ph.D., Biomedical Sciences, University of Tennessee at Knoxville B.A., Physics and Mathematics, Hastings College Dr. Helmenstine holds a Ph.D. in biomedical sciences and is a science writer, educator, and consultant. She has taught science courses at the high school, college, and graduate levels. Learn about our Editorial Process Updated on January 24, 2020 Nucleotides are the building blocks of the DNA and RNA used as genetic material. Nucleotides also are used for cell signaling and to transport energy throughout cells. You may be asked to name the three parts of a nucleotide and explain how they are connected or bonded to each other. Here's the answer for both DNA and RNA. Nucleotides in DNA and RNA Both deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are made up of nucleotides which consist of three parts: Nitrogenous BasePurines and pyrimidines are the two categories of nitrogenous bases. Adenine and guanine are purines. Cytosine, thymine, and uracil are pyrimidines. In DNA, the bases are adenine (A), thymine (T), guanine (G), and cytosine (C). In RNA, the bases are adenine, guanine, uracil, and cytosine. Pentose SugarIn DNA, the sugar is 2'-deoxyribose. In RNA, the sugar is ribose. Both ribose and deoxyribose are 5-carbon sugars. The carbons are numbered sequentially, to help keep track of where groups are attached. The only difference between them is that 2'-deoxyribose has one less oxygen atom attached to the second carbon. Phosphate GroupA single phosphate group is PO43-. The phosphorus atom is the central atom. One atom of oxygen is connected to the 5-carbon in the sugar and to the phosphorus atom. When phosphate groups link together to form chains, as in ATP (adenosine triphosphate), the link looks like O-P-O-P-O-P-O, with two additional oxygen atoms attached to each phosphorus, one on either side of the atom. Although DNA and RNA share some similarities, they are built from slightly different sugars, plus there is a base substitution between them. DNA uses thymine (T), while RNA uses uracil (U). Both thymine and uracil bind to adenine (A). How Are the Parts of a Nucleotide Connected or Attached? The base is attached to the primary or first carbon. The number 5 carbon of the sugar is bonded to the phosphate group. A free nucleotide may have one, two, or three phosphate groups attached as a chain to the 5-carbon of the sugar. When nucleotides connect to form DNA or RNA, the phosphate of one nucleotide attaches via a phosphodiester bond to the 3-carbon of the sugar of the next nucleotide, forming the sugar-phosphate backbone of the nucleic acid. Cite this Article Format mla apa chicago Your Citation Helmenstine, Anne Marie, Ph.D. "What Are the 3 Parts of a Nucleotide? How Are They Connected?" ThoughtCo, Jul. 29, 2021, thoughtco.com/what-are-the-parts-of-nucleotide-606385. Helmenstine, Anne Marie, Ph.D. (2021, July 29). What Are the 3 Parts of a Nucleotide? How Are They Connected? Retrieved from https://www.thoughtco.com/what-are-the-parts-of-nucleotide-606385 Helmenstine, Anne Marie, Ph.D. "What Are the 3 Parts of a Nucleotide? How Are They Connected?" ThoughtCo. https://www.thoughtco.com/what-are-the-parts-of-nucleotide-606385 (accessed February 2, 2023). copy citation Watch Now: What Is DNA?